Enterobacteria resistant to third-generation cephalosporins (3GC-R) and producing extended-spectrum beta-lactamases (ESBLs), initially considered strictly nosocomial, are now increasingly being isolated in the community. Few carriage studies have been conducted in children, particularly in daycare centers, where frequent exposure to antibiotics could promote such carriage. Stool samples collected between January and April 2012 from 419 children in 25 daycare centers in the Alpes-Maritimes region revealed a prevalence rate of 11.2% for C3G-R Enterobacteriaceae and 6.7% for ESBL-producing strains. Clonal spread was observed in one daycare center. Children under 24 months of age and those who had received antibiotic treatment within the previous three months were associated with carriage of C3G-R Enterobacteriaceae. Carriage of ESBL-producing E. coli is not limited to young children attending daycare centers, who constitute a potential reservoir for these strains. (R.A.)