Introduction: Dermatophytoses are frequently diagnosed in childcare settings, environments conducive to epidemic spread due to close contact, the risk of transmission to siblings, and a period of asymptomatic carriage. Materials and Methods: Following the report of a dermatophytosis case in a teacher in Côte-d’Or, a clinical, epidemiological, and biological investigation was initiated. Consequently, a clinical examination and mycological screening of the scalp were offered to the children and staff of the preschool. Follow-up of positive cases and their close contacts was established to assess compliance with the proposed management protocol and adherence to treatment. Results: On clinical examination, 9 children presented with suspicious lesions. Among the 93 children tested, 14 (15%) tested positive for Trichophyton tonsurans: 7 with lesions and 7 without apparent symptoms. Ten children received treatment. Among the 37 identified contacts, 13 (35%) were examined, and 4 (11%) were placed on treatment. Discussion and Conclusion: Screening was well accepted. Not all treatments followed the recommended regimen, but no new cases were detected during follow-up visits conducted by the school health service of the Academic Inspectorate at the preschool, nor were any reported to the Regional Health Agency since the implementation of hygiene measures and treatments. This investigation confirmed the value of combining clinical examination with mycological screening to curb dermatophytoses occurring in child care settings. (R.A.)