The full questionnaire for the 2021 Santé publique France Public Health Barometer is presented here. It includes the instructions displayed on the interviewers’ screens as well as the wording of the questions. Topics covered include: pesticides, environmental health, risks associated with natural UV radiation, tobacco, cannabis, alcohol, vaccination, diet, dietary recommendations, Nutri-Score, general health, healthcare utilization and non-utilization, diabetes, high blood pressure, COVID-19, lockdown, mental health, physical activity and sedentary behavior, musculoskeletal disorders (MSDs), diet, antibiotic resistance, activities with a risk of accidents...