Introduction. Surveillance of the diversity of HIV, HBV, and HCV viruses in blood donors (BDs) is conducted at the laboratory affiliated with the National Reference Centers (NRCs) for hepatitis B and C and HIV at the National Blood Transfusion Institute (INTS). The study period spans from 2000 to 2010. Methods. Plasma samples from all BD infected with the viruses under consideration are centralized at the INTS. Plasma viral loads, genotyping via sequencing of various genomic regions, and phenotyping via serotype determination are the primary tools used for viral characterization. Results. The proportion of non-B HIV-1 group M serotypes increased steadily to exceed 30% during the 2007–2010 period. The predominant HIV-1 genotype is genotype B (66.7%), followed by genotype CRF02_AG (19.0%). The distribution of HBV genotypes is as follows: D (42.4%), A (27.2%), E (16.8%), B (6.3%), C (6.5%), and F (0.7%). Genotype A1, of African origin, is more common in the Caribbean. The most common HCV genotype is genotype 1 (57.4%) (51.2% 1b and 48.4% 1a), followed by genotypes 3 (21%), 2 (11.5%), and 4 (8.7%). The genotypic distributions of HBV and HCV are relatively stable. Conclusion. The most common genotypes infecting people living with HIV/HBV/HCV are: genotype B for HIV, genotype D for HBV, and genotype 1b for HCV, with a genotypic trend marked primarily, for HIV, by an increase in non-B strains. These observations are consistent with the spread of these viruses in the French population and the risk factors identified among the study subjects. (R.A.)