Introduction: The pilot phase of the Coset-MSA program assessed the prevalence of upper-limb musculoskeletal symptoms among agricultural workers, particularly among farm owners and farm laborers covered by the Mutualité sociale agricole (MSA). Method: Workers were recruited in five departments through random selection from the MSA databases and invited to complete a self-administered questionnaire. The analysis sample consisted of 1,130 workers, aged 18 to 65, employed in the agriculture, forestry, and fishing sector, and currently working at the time of the survey. Results: The prevalence of reported upper limb symptoms over the past 12 months was 54% among men and 67% among women (p<0.03), and 20% and 34%, respectively, had symptoms lasting more than 30 days. The highest prevalence over the past 12 months was observed in the shoulder for men (37%) and the hand/wrist area for women (49%). Women had significantly higher prevalence rates than men, except for the elbow/forearm, where no difference was observed. The prevalence of symptoms did not differ significantly between farm owners and farm workers, among both men and women. Discussion and conclusion: This study shows a high prevalence of upper limb musculoskeletal symptoms among agricultural workers, who constitute a population that requires particular monitoring and should be targeted for preventive measures.